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Rudina Bleta

PhD from Nancy University and postdoctoral studies from University Paul Sabatier at the CIRIMAT-Carnot

Title: Immobilization of Candida Antarctica lipase B in a silicified hydrogel support and its application as bioreactor

Biography

Biography: Rudina Bleta

Abstract

Supramolecular hydrogels have attracted increasing interest in recent years because of their ability to incorporate high levels of proteins, cells, antibodies, peptides and genes [1-2]. In this work, we propose a new approach to confinement of Candida Antarctica lipase B (CALB) within a supramolecular silicified hydrogel based on Pluronic F127 and α-cyclodextrin (α-CD) [3]. After functionalization of the matrix, the catalytic performance of the supported biocatalyst was evaluated in the oxidation of 2,5-diformylfuran (DFF) to 2,5-furandicarboxylic acid (FDCA), a fully biosourced alternative to terephthalic acid used in the production of polyethylene terephthalate (PET) [4]. Our results revealed that while CALB immobilized in conventional sol-gel silica yielded exclusively 5-formylfuran-2-carboxylic acid (FFCA), confinement of the enzyme in the silicified hydrogel imparted a 5-fold increase in DFF conversion and afforded 67% FDCA yield in 7 h and almost quantitative yields in less than 24 h. The hierarchically interconnected pore structure of the host matrix was found to provide a readily accessible diffusion path for reactants and products, while its flexible hydrophilic-hydrophobic interface was extremely beneficial for the interfacial activation of the immobilized lipase